Investigation of the retention behaviour of indole derivatives in reversed phase TLC depending on structure and eluent.
Summary
Type: TLC Application #: 400700
Detection: Spray with 1% p-dimethylaminobenzaldehyde in conc. hydrochloric acid/methanol (1:1), then heat 20 min to 50 C Conditions: Eluent: several eluents were investigated: A) water / methanol / acetic acid (59:40:1, v/v) B) 0.1 M ammonia in 40% methanol C) hexane / ethyl acetate / acetic acid (72:27:1, v/v) D) 0.1 M ammonia + 0.1 M ammonium chloride in 40% methanol E) 0.5 M ammonia + 0.5 M ammonium chloride in 40% methanol F) hexane / ethyl acetate / acetic acid (67:32:1, v/v) Authors: Lepri L Source: J Chromatogr 260 (1983) 383